Addgene - pMXs-Utf1 Plasmid Data

Gene/insert name:		undifferentiated embryonic cell transcription factor 1
Alternative names:		Utf1
Insert size (bp):		1020
Gene/insert aliases:		Utf1, AI505934
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		None
Vector backbone:		pMXs (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		4600
5' Sequencing primer:		pMX-S1811 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Shinya Yamanaka
Terms and Licenses:		MTA

Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K et al. (Cell. 2006 Aug 25. 126(4):663-76. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13369" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.