Addgene - pLDLR-Luc (aka: pES7) Plasmid Data

Gene/insert name:		LDLR promoter
Alternative names:		LDL receptor promoter
		LDL receptor
Insert size (bp):		340
GenBank/Entrez ID of insert:		NC_000019
Gene/insert aliases:		LDLR, FH, FHC, LDLCQ2
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		luciferase
Terminal:		C terminal on backbone
Vector backbone:		pGL2 basic (Search Vector Database)
Backbone manufacturer:		Promega
Type of vector:		Mammalian expression,Luciferase
Backbone size (bp):		5597
Cloning site 5':		SacI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		n/a (List of Sequencing Primers)
3' Sequencing primer:		LucNrev
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Axel Nohturfft
Terms and Licenses:		MTA, Luciferase LULL

Comments: To generate pLDLR-Luc, a PCR fragment containing nucleotides –335 to +3 of the human LDL receptor gene was cloned into pGL2-basic.

PCR'd using primers: CGATCCTCTACTGACCTC and GGCCCACGTCATTTACAGCATTTC.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

LucNrev_primer

526 - 508

firefly_Luciferase

442 - 2073

ORF frame 1

442 - 2094

SV40_int

2334 - 2349

SV40_3_splice

2355 - 2402

SV40_PA_terminator

2978 - 3109

EBV_rev_primer

3066 - 3085

pBR322_origin

4026 - 3407

ORF frame 2

5041 - 4181

Ampicillin

5041 - 4181

AmpR_promoter

5111 - 5083

f1_origin

5190 - 5496

SV40_PA_terminator

5821 - 5940

EBV_rev_primer

5909 - 5928

Unique restriction sites

SmaI		3
KpnI		12
SacI		18
NheI		28
EagI		269
XhoI		399
BglII		403
HindIII		413
NarI		475
XbaI		489
EcoRI		1029
EcoRV		1780
ClaI		1807
BamHI		3105
SalI		3111

Article: Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Castoreno AB et al. (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14940" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.