Addgene - pHMGCS-Luc (aka: pES8) Plasmid Data

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AH001829

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pLDLR-Luc ( aka: pES7)

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Plasmid 14947: pHMGCS-Luc (aka: pES8)

Gene/insert name:		HMG CoA synthase promoter
Alternative names:		3-hydroxy-3-methylglutaryl CoA synthase
		HMGCS
Insert size (bp):		400
GenBank/Entrez ID of insert:		AH001829
Species of gene(s):		hamster
Fusion proteins or tags:		luciferase
Terminal:		C terminal on backbone
Vector backbone:		pGL2 basic (Search Vector Database)
Backbone manufacturer:		Promega
Type of vector:		Mammalian expression,Luciferase
Backbone size (bp):		5597
Cloning site 3':		HindIII
Site destroyed during cloning:		No
5' Sequencing primer:		n/a (List of Sequencing Primers)
3' Sequencing primer:		LucNrev
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Axel Nohturfft
Terms and Licenses:		MTA, Luciferase LULL

Comments: To generate pHMGCS-Luc, a PCR fragment containing nucleotides –379 to –17 of the hamster 3-hydroxy-3-methylglutaryl (HMG) CoA synthase gene was cloned into pGL2-basic.

PCR'd using primers: CGATCTCCTCTCACTGACCTTC and GCAGCTTTATAGCCACCGACCC.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

LucNrev_primer

545 - 527

firefly_Luciferase

461 - 2092

ORF frame 2

461 - 2113

SV40_int

2353 - 2368

SV40_3_splice

2374 - 2421

SV40_PA_terminator

2997 - 3128

EBV_rev_primer

3085 - 3104

pBR322_origin

4045 - 3426

ORF frame 3

5060 - 4200

Ampicillin

5060 - 4200

AmpR_promoter

5130 - 5102

f1_origin

5209 - 5515

SV40_PA_terminator

5840 - 5959

EBV_rev_primer

5928 - 5947

Unique restriction sites

KpnI		397
NheI		413
XhoI		418
BglII		422
HindIII		432
NarI		494
XbaI		508
EcoRI		1048
EcoRV		1799
ClaI		1826
BamHI		3124
SalI		3130

Article: Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Castoreno AB et al. (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14947" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.