Addgene - pCMV Fcgamma RIIa IRES neo Plasmid Data

Browse > Axel Nohturfft > Trivedi et al > pCMV Fcgamma RIIa IRES neo

Price: $65.00

Plasmid Links

Related Plasmids

Axel Nohturfft Lab Plasmids

Other Links

NCBI: FCGR2A

FCGR2A antibodies

Print Friendly Email

Plasmid 14948: pCMV Fcgamma RIIa IRES neo

Gene/insert name:		Fcgamma RIIa
Alternative names:		FcgRIIa
		Fc gamma RIIa
Insert size (bp):		1000
Gene/insert aliases:		FCGR2A, CD32, FCG2, FcGR, CD32A, CDw32, FCGR2, IGFR2, FCGR2A1, MGC23887, MGC30032
Species of gene(s):		H. sapiens (human)
Vector backbone:		pIRESneo2 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		5300
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		NotI
Site destroyed during cloning:		No
5' Sequencing primer:		CMV-F (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Axel Nohturfft
Terms and Licenses:		MTA

Comments: The FcgRIIa cDNA was amplified by RT-PCR using a Titan One Tube RT-PCR System kit (Roche, Mannheim, Germany) and a pair of primers (5'-TTGAATTCACCATGGAGACCCAAATGTCTC-3' and 5'-GCGGCCGCTTAGTTATTACTGTTGACATGG-3'). The PCR product was cloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA) to generate pAC01 and subcloned as an EcoRI–NotI fragment into pIRESneo2 (Clontech, Mountain View, CA) to generate pCMV-FcgRIIa-IRES-Neo.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

315 - 602

CMV_immearly_promoter

236 - 852

CMV_fwd_primer

769 - 789

T7_promoter

863 - 881

ORF frame 2

980 - 1924

synth_int

1957 - 2252

IRES

2279 - 2842

ORF frame 3

2763 - 3692

NeoR/KanR

2889 - 3689

BGH_rev_primer

3741 - 3724

bGH_PA_terminator

3727 - 3954

EBV_rev_primer

4001 - 4020

M13_reverse_primer

4094 - 4076

M13_pUC_rev_primer

4115 - 4093

lac_promoter

4158 - 4129

pBR322_origin

5086 - 4467

ORF frame 3

6101 - 5241

Ampicillin

6101 - 5241

AmpR_promoter

6171 - 6143

Unique restriction sites

NruI		208
SpeI		249
NdeI		484
ClaI		910
EcoRV		914
NheI		927
AflII		934
StuI		940
HpaI		946
AgeI		948
BstBI		969
EcoRI		971
BamHI		1474
NotI		1926
SacII		2083
ApaI		2397
SmaI		2867
NarI		3026
XbaI		3705
BclI		3717
XhoI		3983

Article: Immunoglobulin G signaling activates lysosome/phagosome docking. Trivedi V et al. (Proc Natl Acad Sci U S A. 2006 Nov 28. 103(48):18226-31. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14948" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.