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Browse > Linzhao Cheng > Yu et al > EF.mHES1.CMV.GFP
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Hes1 plasmids
Linzhao Cheng Lab Plasmids
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NCBI: Hes1
 

This is commonly
requested with
EF.hICN1.CMV. GFP
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Plasmid 17622: EF.mHES1.CMV.GFP
Gene/insert name: HES1
Alternative names: mHES1
Hes-1
Insert size (bp): 850
Gene/insert aliases: Hes1, Hry, bHLHb39
Species of gene(s): M. musculus (mouse)
Vector backbone: EF.v-CMV.GFP
(Search Vector Database)
Backbone manufacturer: Linzhao Cheng Lab
Type of vector: Mammalian expression,Lentiviral
Backbone size (bp): 8200
5' Sequencing primer: EF-1a Forward  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: Unknown
Grow in standard E. coli @ 37C: Yes
Selectable markers: GFP
Sequence:View sequence
Plasmid Provided In:Stbl3
Principal Investigator:Linzhao Cheng
Terms and Licenses:MTA

Comments: The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. mHES1 was cloned downstream of the EF-1a promoter.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
AmpR_promoter 62 - 90
ORF frame 3 132 - 992
Ampicillin 132 - 992
pBR322_origin 1147 - 1766
lac_promoter 2075 - 2104
M13_pUC_rev_primer 2118 - 2140
M13_reverse_primer 2139 - 2157
T3_promoter 2174 - 2193
HIV-1_5_LTR 2476 - 2656
truncHIV-1_3_LTR 2476 - 2656
HIV-1_psi_pack 2764 - 2808
RRE 3318 - 3551
EF1a_promoter 4164 - 5347
ORF frame 3 5087 - 4467
EF1a_fwd_primer 5295 - 5315
T7_promoter 5364 - 5382
ORF frame 2 5417 - 6265
CAG_enhancer 6347 - 6634
CMV_immearly_promoter 6292 - 6844
CMV_fwd_primer 6801 - 6821
CMV_promoter 6802 - 6871
EGFP_N_primer 7004 - 6983
EGFP 6938 - 7654
ORF frame 2 6938 - 7657
EGFP_C_primer 7591 - 7612
cPPT 7738 - 7753
U3PPT 7738 - 7759
delta_U3 7755 - 7807
HIV-1_5_LTR 7808 - 7988
truncHIV-1_3_LTR 7808 - 7988
SV40_PA_terminator 8063 - 8182
EBV_rev_primer 8151 - 8170
SV40_origin 8200 - 8277
SV40pro_F_primer 8262 - 8281
T7_promoter 8386 - 8368
M13_forward20_primer 8412 - 8396
M13_pUC_fwd_primer 8427 - 8405
lacZ_a 8405 - 8548
f1_origin 8574 - 8880
Unique restriction sites

NruI 2852
PmlI 5796
HpaI 5809
MscI 5846
NdeI 6516
NheI 6873
BstBI 6909
BamHI 6919
SalI 7667

Article: HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X et al. (Stem Cells. 2006 Apr . 24(4):876-88. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 17622" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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