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Browse > Konrad Hochedlinger > Maherali et al > FU-tet-o-hKLF4
Price: $65.00
 

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KLF4 plasmids
Konrad Hochedlinger Lab Plasmids
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NCBI: KLF4
 

This is commonly
requested with
FU-tet-o-hOct4
FU-tet-o-hSox2
FU-tet-o-hc- myc
FUdeltaGW-rtTA
FU-tet-o-hNanogP8
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Plasmid 19777: FU-tet-o-hKLF4
Gene/insert name: Klf4
Insert size (bp): 1425
Gene/insert aliases: KLF4, EZF, GKLF
Species of gene(s): H. sapiens (human)
Vector backbone: FUdeltaGW
(Search Vector Database)
Type of vector: Mammalian expression,Lentiviral
Backbone size (bp): 8400
Cloning site 5': EcoRI
Site destroyed during cloning: Unknown
Cloning site 3': EcoRI
Site destroyed during cloning: Unknown
5' Sequencing primer: CMV-F  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Stbl3
Sequence:View sequence
Plasmid Provided In:Stbl3
Principal Investigator:Konrad Hochedlinger
Terms and Licenses:MTA, Tet Systems LULL

Comments: From article, concerning viral infections: For a standard infection in a 35 mmdish (aprox 10^5 cells), 10 microL rtTA+5 microL factors (OCT4, SOX2, KLF4, and NANOG)+2 microL cMYC was used in an overnight infection supplemented with 6 microg/microL polybrene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
CAG_enhancer 318 - 605
CMV_immearly_promoter 239 - 815
CMV_fwd_primer 772 - 792
HIV-1_5_LTR 835 - 1015
truncHIV-1_3_LTR 835 - 1015
HIV-1_psi_pack 1126 - 1170
RRE 1680 - 1913
ORF frame 1 1558 - 2445
cPPT 2444 - 2459
TRE 2608 - 2913
pCEP_fwd_primer 2945 - 2964
LNCX_primer 2947 - 2971
CMV2_promoter 2914 - 3033
ORF frame 2 3056 - 4468
ORF frame 2 4264 - 3413
WPRE 4495 - 5082
pBluescriptKS_primer 5101 - 5085
ORF frame 2 4583 - 5632
cPPT 5269 - 5284
U3PPT 5269 - 5290
HIV-1_5_LTR 5606 - 5786
truncHIV-1_3_LTR 5606 - 5786
BGH_rev_primer 5829 - 5812
bGH_PA_terminator 5815 - 6042
f1_origin 6105 - 6411
pBABE_3_primer 6545 - 6525
SV40_enhancer 6746 - 6531
SV40_promoter 6543 - 6811
SV40_origin 6710 - 6787
SV40pro_F_primer 6772 - 6791
EM7_promoter 6905 - 6972
bleo 6973 - 7344
sh_ble 6973 - 7347
SV40_PA_terminator 7480 - 7599
EBV_rev_primer 7568 - 7587
M13_reverse_primer 7661 - 7643
M13_pUC_rev_primer 7682 - 7660
lac_promoter 7725 - 7696
pBR322_origin 8653 - 8034
ORF frame 3 9668 - 8808
Ampicillin 9668 - 8808
AmpR_promoter 9738 - 9710
Unique restriction sites

SpeI 252
NdeI 487
NotI 1525
XhoI 5097
FspI 9103

Article: A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N et al. (Cell Stem Cell. 2008 Sep 11. 3(3):340-5. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 19777" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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