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Browse > John Ohlfest > Wiesner et al > pT/CMV-SV40-LgT
Price: $65.00
 

 

This is commonly
requested with
PT2/C-fluc
pT/Caggs-NRASV12
Plasmid 20204: pT/CMV-SV40-LgT
Gene/insert name: SV40LgT
Insert size (bp): 2473
Species of gene(s): M. musculus (mouse)
viral
Vector backbone: colE1/ampR
(Search Vector Database)
Backbone manufacturer: Ohlfest Lab
Type of vector: Transposon
Backbone size (bp): 4663
5' Sequencing primer: n/a  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Please specify bacterial strain for growth and growth condition: DH5-alpha
Sequence:View sequence
Author's Map:View map
Plasmid Provided In:DH5a
Principal Investigator:John Ohlfest
Terms and Licenses:MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
BGH_rev_primer 24 - 7
bGH_PA_terminator 10 - 237
M13_reverse_primer 683 - 665
M13_pUC_rev_primer 704 - 682
lac_promoter 747 - 718
pBR322_origin 1675 - 1056
ORF frame 3 2690 - 1830
Ampicillin 2690 - 1830
AmpR_promoter 2760 - 2732
pGEX_3_primer 2941 - 2919
lacZ_a 3288 - 3140
M13_pUC_fwd_primer 3254 - 3276
M13_forward20_primer 3269 - 3285
CAG_enhancer 3764 - 4051
CMV_immearly_promoter 3685 - 4285
CMV_fwd_primer 4218 - 4238
T7_promoter 4313 - 4331
SV40_int 5006 - 5021
SV40_3_splice 5027 - 5074
SV40_lrgT 5085 - 6953
ORF frame 3 5151 - 6953
SV40_PA_terminator 6979 - 7110
EBV_rev_primer 7067 - 7086
Unique restriction sites

XbaI 627
SalI 633
NarI 3126
NcoI 4059
NotI 4415
BclI 6869

Article: De novo induction of genetically engineered brain tumors in mice using plasmid DNA. Wiesner SM et al. (Cancer Res. 2009 Jan 15. 69(2):431-9. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 20204" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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