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Browse > James A Thomson > Yu et al > pEP4 E02S EN2L
Price: $65.00
 

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NM_002701
NM_003106
NM_024865
NM_024674
 

This is commonly
requested with
pEP4 E02S EM2K
pEP4 E02S ET2K
pEP4 E02S EN2K
pEP4 E02S CK2M EN2L
pCEP4-M2L
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Plasmid 20922: pEP4 E02S EN2L
Gene/insert name: Oct4 and Sox2; Nanog and Lin28
Insert size (bp): 4900
GenBank/Entrez ID of insert: NM_002701 NM_003106 NM_024865 NM_024674
Species of gene(s): H. sapiens (human)
Vector backbone: pCEP4
(Search Vector Database)
Backbone manufacturer: Invitrogen
Type of vector: Episomal/EBNA
Backbone size (bp): 10500
Cloning site 5': multiple
Site destroyed during cloning: Unknown
Cloning site 3': multiple
Site destroyed during cloning: Unknown
5' Sequencing primer: na  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Top10 "Extremely" low copy number
Sequence:View sequence
Author's Map:View map
Plasmid Provided In:Top10F'
Principal Investigator:James A Thomson
Terms and Licenses:MTA

Comments: Oct4 and Sox2 are coexpressed under a strong EF promoter. Nanog and Lin28 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells.

These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
ORF frame 1 915 - 1
EF1a_promoter 8 - 1175
EF1a_fwd_primer 1123 - 1143
ORF frame 2 1229 - 2311
IRES 2384 - 2968
ORF frame 3 2868 - 3944
EBV_rev_primer 4020 - 4001
SV40_PA_terminator 4106 - 3987
pGEX_3_primer 4276 - 4254
ORF frame 3 5543 - 4629
EF1a_promoter 4636 - 5803
EF1a_fwd_primer 5751 - 5771
ORF frame 1 5872 - 6789
IRES 6846 - 7430
ORF frame 2 7457 - 8086
EBV_rev_primer 8159 - 8140
SV40_PA_terminator 8245 - 8126
pGEX_3_primer 8415 - 8393
EBV_origin 10761 - 8602
ORF frame 2 12997 - 11072
ORF frame 1 12882 - 11770
ORF frame 1 11785 - 12999
AmpR_promoter 13557 - 13585
ORF frame 1 13627 - 14487
Ampicillin 13627 - 14487
pBR322_origin 14642 - 15261
Unique restriction sites

BclI 3808
SalI 4371
BglII 4461
NotI 6808
NruI 7439
XbaI 7888
EcoRV 9113
AatII 13495
NheI 15415

Article: Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Yu J et al. (Science. 2009 Mar 26. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 20922" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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