pQCXIH CMV/TO SV40 Large T K1 mutant (w417-1)
(Plasmid #22273)

• Depositing Lab: Eric Campeau
• Publication: shMDC1 plasmids (unpublished)

Backbone

• Vector backbone: pQCXIH
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7911
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SV40 Large T antigen K1 mutant
• Alt name: Large T K1
• Insert Size (bp): 2126
• Mutation: E107K (K1 mutation)
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMVforw
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Large T K1 mutant was obtained from Dr. Francis Rodier.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Large T antigen K1 mutant can no longer bind and inactivate the Rb, p130 and p107 proteins (DeCaprio et al. 1988, Stubdal et al. 1997). It also fails to activate Akt (Yu and Alwine 2008).