Addgene - pDEST26-hOPTN Plasmid Data

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Plasmid 23050: pDEST26-hOPTN

Gene/insert name:		Optineurin
Insert size (bp):		1733
Gene/insert aliases:		OPTN, NRP, FIP2, HIP7, HYPL, GLC1E, TFIIIA-INTP
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		N/A
Fusion proteins or tags:		His
Terminal:		N terminal on backbone
Vector backbone:		pDEST26 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		5800
Cloning site 5':		attR
Site destroyed during cloning:		Unknown
Cloning site 3':		attR
Site destroyed during cloning:		Unknown
5' Sequencing primer:		CMVPro Fwd (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Jon Ashwell
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Zhu G et al. (Curr Biol. 2007 Aug 21. 17(16):1438-43. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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