Addgene - EF.STAT3C.Ubc.GFP Plasmid Data

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Plasmid 24983: EF.STAT3C.Ubc.GFP

Gene/insert name:		STAT3
Insert size (bp):		2300
Gene/insert aliases:		STAT3, APRF, HIES, FLJ20882, MGC16063
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		C661A, C663N
Fusion proteins or tags:		FLAG
Terminal:		C terminal on insert
Vector backbone:		cFUGW (Search Vector Database)
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		11500
Cloning site 5':		PacI
Site destroyed during cloning:		Unknown
Cloning site 3':		unknown
Site destroyed during cloning:		Unknown
5' Sequencing primer:		EF1a-forward (List of Sequencing Primers)
3' Sequencing primer:		hUBCpro-R
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Stbl 3
Sequence:		View sequence
Plasmid Provided In:		Stbl3
Principal Investigator:		Linzhao Cheng
Terms and Licenses:		MTA

Comments: An L666V mutation is present but not known to affect function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: The high-mobility group A1a/signal transducer and activator of transcription-3 axis: an achilles heel for hematopoietic malignancies? Hillion J et al. (Cancer Research 68, 10121, December 15, 2008. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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