-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 25433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepQCXI
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7072
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDEST cassette from pcDNADEST40
-
Insert Size (bp)1900
-
MutationpQCXI retroviral backbone, in which Eric Campeau replaced the antibiotic resistance gene with DsRed2 fluorescent protein. Next, the Gateway DEST cassette from pcDNADEST40 was inserted into the Age I site. This cassette includes the original V5, His tags at C-terminal end, and both can be used to tag proteins
-
Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site Age I (not destroyed)
- 3′ cloning site Age I (not destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThe plasmid pQCXIR was received from Eric Campeau. The DEST cassette was cloned into the plasmid in our lab by by Octavian Henegariu, M.D.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Any mismatches between Addgene QC sequenced and the depositing lab's sequence is inconsequential.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pQCXIR-DEST was a gift from Kevan Herold (Addgene plasmid # 25433)