| Gene/insert name: | | Yeast Gal4 | |
| Insert size (bp): | | 3000 | |
| GenBank/Entrez ID of insert: | | K01486 | |
| Gene/insert aliases: | | GAL4, GAL81 | |
| Species of gene(s): | | S. cerevisiae (budding yeast)
fused to first ~30 amino acids of Drosophila ADH
| |
| Vector backbone: | | pCasper
(Search Vector Database) | |
| Backbone manufacturer: | | Public Domain--Carl Thummel | |
| Type of vector: | | Drosophila P-element vector | |
| Backbone size (bp): | | 9000 | |
| Cloning site 5': | | EcoRI, Asp718I*, NotI, BamHI, BglII, PmeI, Asp718* | |
| Site destroyed during cloning: | | No | |
| 5' Sequencing primer: | | gggttttattaacttacat (List of Sequencing Primers) | |
| 3' Sequencing primer: | | taagtcggcaaatatcgc | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | Unknown | |
| Grow in standard E. coli @ 37C: | | Yes | |
| If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: | | Plasmid was constructed by deleting B-Gal gene from pCasperAUG-Bgal from Carl Thummel/Univ. of Utah and replacing with the coding region of Gal4 from the GL83 plasmid provided by Gary Struhl/Columbia University | |
| Sequence: | | View sequence |
| Author's Map: | | View map |
| Plasmid Provided In: | | DH5a |
| Principal Investigator: | | Leslie B. Vosshall |
| Terms and Licenses: | | MTA |
Article: An olfactory sensory map in the fly brain. Vosshall LB et al. (Cell 2000 Jul 21;102(2):147-59.
Pubmed)
Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.
Also, please include the text "Addgene plasmid 8378" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.
