| Gene/insert name: | | Protein Kinase B | |
| Alternative names: | | Akt1 | |
| | PKB | |
| | PKBalpha | |
| Insert size (bp): | | 1500 | |
| GenBank/Entrez ID of insert: | | NC_000078 | |
| Gene/insert aliases: | | Akt1, Akt, PKB, Rac, PKB/Akt, PKBalpha | |
| Species of gene(s): | | M. musculus (mouse)
| |
| Fusion proteins or tags: | | myr signal | |
| Terminal: | | N terminal on insert | |
| Fusion proteins or tags: | | HA tag | |
| Terminal: | | N terminal on insert | |
| Vector backbone: | | RCAS
(Search Vector Database) | |
| Type of vector: | | Retroviral | |
| Backbone size (bp): | | 11600 | |
| Cloning site 5': | | Pme1 | |
| Site destroyed during cloning: | | Yes | |
| Cloning site 3': | | Pme1 | |
| Site destroyed during cloning: | | Yes | |
| 5' Sequencing primer: | | RCAS-F (List of Sequencing Primers) | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | Unknown | |
| Grow in standard E. coli @ 37C: | | Yes | |
| Sequence: | | Visit www.addgene.org/11547 | | Plasmid Provided In: | | DH5a | | Principal Investigator: | | Sandra Orsulic | Comments: The RCAS vectors that carry oncogenes are harmless for mammalian cells, but can be oncogenic in avian cells since some avian cells express the receptor for the RCAS virus. Article: Induction of ovarian cancer by defined multiple genetic changes in a mouse model system. Orsulic S et al. (Cancer Cell. 2002 Feb . 1(1):53-62. Pubmed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11547" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication. |
