| Gene/insert name: | | F' Tra operon | |
| Insert size (bp): | | Unknown | |
| Vector backbone: | | na
(Search Vector Database) | |
| Type of vector: | | Bacterial expression | |
| Backbone size (bp): | | Unknown | |
| 5' Sequencing primer: | | na (List of Sequencing Primers) | |
| Bacteria resistance: | | Tetracycline | |
| High or low copy: | | Low Copy | |
| Grow in standard E. coli @ 37C: | | Yes | |
| Type of Material: | | Bacterial Strain | | Principal Investigator: | | Stephen Elledge | | Additional Documents: | | Elledge-pPRIME (PDF)
| Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt. Article: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier F et al. (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. Pubmed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12082" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication. | 