| Gene/insert name: | | mU6 promoter | |
| Insert size (bp): | | 707 | |
| GenBank/Entrez ID of insert: | | X06980 | |
| Vector backbone: | | pGEM-T Easy
(Search Vector Database) | |
| Backbone manufacturer: | | Promega | |
| Type of vector: | | Bacterial expression | |
| Backbone size (bp): | | 3015 | |
| Cloning site 5': | | PCR | |
| Site destroyed during cloning: | | Unknown | |
| Cloning site 3': | | PCR | |
| Site destroyed during cloning: | | Unknown | |
| 5' Sequencing primer: | | SP6 (List of Sequencing Primers) | |
| 3' Sequencing primer: | | T7 | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | High Copy | |
| Grow in standard E. coli @ 37C: | | Yes | |
| Sequence: | | Visit www.addgene.org/12116 | | Plasmid Provided In: | | DH5a | | Principal Investigator: | | Inder M Verma | Article: CRE recombinase-inducible RNA interference mediated by lentiviral vectors. Tiscornia G et al. (Proc Natl Acad Sci U S A. 2004 May 11. 101(19):7347-51. Pubmed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12116" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication. | 