| Gene/insert name: | | LDLR promoter | |
| Alternative names: | | LDL receptor promoter | |
| | LDL receptor | |
| Insert size (bp): | | 340 | |
| GenBank/Entrez ID of insert: | | NC_000019 | |
| Gene/insert aliases: | | LDLR, FH, FHC, LDLCQ2 | |
| Species of gene(s): | | H. sapiens (human)
| |
| Fusion proteins or tags: | | luciferase | |
| Terminal: | | C terminal on backbone | |
| Vector backbone: | | pGL2 basic
(Search Vector Database) | |
| Backbone manufacturer: | | Promega | |
| Type of vector: | | Mammalian expression,Luciferase | |
| Backbone size (bp): | | 5597 | |
| Cloning site 5': | | SacI | |
| Site destroyed during cloning: | | No | |
| Cloning site 3': | | XhoI | |
| Site destroyed during cloning: | | No | |
| 5' Sequencing primer: | | n/a (List of Sequencing Primers) | |
| 3' Sequencing primer: | | LucNrev | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | High Copy | |
| Grow in standard E. coli @ 37C: | | Yes | |
| Sequence: | | Visit www.addgene.org/14940 | | Author's Map: | | Visit www.addgene.org/14940 | | Plasmid Provided In: | | DH5a | | Principal Investigator: | | Axel Nohturfft | Comments: To generate pLDLR-Luc, a PCR fragment containing nucleotides –335 to +3 of the human LDL receptor gene was cloned into pGL2-basic. PCR'd using primers: CGATCCTCTACTGACCTC and GGCCCACGTCATTTACAGCATTTC. Article: Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Castoreno AB et al. (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34. Pubmed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14940" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication. | 