Find this plasmid at: www.addgene.org
Enter "22223" in the search box
 
Plasmid 22223: FCK-Mac-GFP
Gene/insert name: Mac
Alternative names: Mac-GFP
Leptosphaeria maculans opsin
opsin
Insert size (bp): 1683
Species of gene(s): L. maculans
Relevant mutations/deletions: NO
Fusion proteins or tags: GFP
Terminal: C terminal on insert
Vector backbone: FCK(1.3)GW
(Search Vector Database)
Backbone manufacturer: Pavel Osten
Type of vector: Mammalian expression,Lentiviral
Backbone size (bp): 9227
Cloning site 5': BamHI
Site destroyed during cloning: No
Cloning site 3': EcoRI
Site destroyed during cloning: No
5' Sequencing primer: atgactgagaccctcccacccgtg actgaaagcgccgt  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: Unknown
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:Visit www.addgene.org/22223
Author's Map:Visit www.addgene.org/22223
Plasmid Provided In:Stbl3
Principal Investigator:Edward Boyden

Please check www.addgene.org/22223 for updated plasmid information and related links.

Page 1 of 3 - Date: 09/09/2010

Information on this datasheet is provided pursuant to Addgene's Terms of Use at www.addgene.org.

Find this plasmid at: www.addgene.org
Enter "22223" in the search box
 
Plasmid 22223: FCK-Mac-GFP
Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Article: High-performance genetically targetable optical neural silencing by light-driven proton pumps. Chow BY et al. (Nature. 2010 Jan 7. 463(7277):98-102. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22223" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

Please check www.addgene.org/22223 for updated plasmid information and related links.

Page 2 of 3 - Date: 09/09/2010

Information on this datasheet is provided pursuant to Addgene's Terms of Use at www.addgene.org.

Find this plasmid at: www.addgene.org
Enter "22223" in the search box
 

Please check www.addgene.org/22223 for updated plasmid information and related links.

Page 3 of 3 - Date: 09/09/2010

Information on this datasheet is provided pursuant to Addgene's Terms of Use at www.addgene.org.