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Human Epigenetic Knockout Library
(Pooled Library #162256)

  • Purpose

    This lentiviral CRISPR knockout library targets human epigenetic genes.

  • Vector Backbone

    lentiCRISPR v2 - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 162256 gRNA pooled library 1 $400 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    2,508
  • gRNAs
    20,051
  • Controls
    50 intergenic region targeting gRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    25ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Epigenetic Knockout Library was a gift from Kivanc Birsoy (Addgene #162256)
  • For your References section:

    ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase. Williams RT, Guarecuco R, Gates LA, Barrows D, Passarelli MC, Carey B, Baudrier L, Jeewajee S, La K, Prizer B, Malik S, Garcia-Bermudez J, Zhu XG, Cantor J, Molina H, Carroll T, Roeder RG, Abdel-Wahab O, Allis CD, Birsoy K. Cell Metab. 2020 Apr 7;31(4):852-861.e6. doi: 10.1016/j.cmet.2020.03.008. PubMed 32268116