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Purpose
This lentiviral CRISPR knockout library targets mouse metabolic genes, including most metabolic enzymes and transporters.
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Vector Backbone
LentiCRISPRv2-Opti - expresses Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 160129 | gRNA pooled library | 1 | $ 430 | Add to Cart | |
Available to Academic and Nonprofits Only
Library Details
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SpeciesMouse
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Genes targeted2,865
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gRNAs22,909
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Controls50 guides targeting intergenic regions
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration25 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
How to cite this pooled library
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These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse CRISPR Metabolic Gene Knockout library was a gift from Kivanc Birsoy (Addgene #160129) -
For your References section:
Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells. Zhu XG, Chudnovskiy A, Baudrier L, Prizer B, Liu Y, Ostendorf BN, Yamaguchi N, Arab A, Tavora B, Timson R, Heissel S, de Stanchina E, Molina H, Victora GD, Goodarzi H, Birsoy K Cell Metab. 2020 Oct 28. pii: S1550-4131(20)30550-7. doi: 10.1016/j.cmet.2020.10.017. PubMed 33152324