Borodina Yeast Transporter Libraries
(Pooled Libraries #153101, 153102, 153103, 153104, 153105, 153106)

• Purpose:

  These yeast pooled CRISPR libraries target all 361 non-essential and conditionally essential transporter genes in S. cerevisiae. Plasmids contain both an sgRNA and corresponding 165 bp donor fragment, achieving high efficiency disruption of the target genes by introduction of frameshift mutations.

  Libraries are divided into five sub-groups targeting different groups of transporters.

  • Sctrans-PP-Grp1: ATP dependent transporters
  • Sctrans-PP-Grp2: Secondary transporters (SeTS-1 to SeTS-5)
  • Sctrans-PP-Grp3: Secondary transporters (SeTS-6 to SeTS-9)
  • Sctrans-PP-Grp4: Secondary transporters (SeTS-10 to SeTS-14)
  • Sctrans-PP-Grp5: Ion channel, unclassified, and other transporters
  • Sctrans-PP targets all 361 genes

• Vector Backbone:

  pMEL10 - does not express Cas9

• Depositing Labs: Irina Borodina
• Publication: Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(21)00015-X. doi: 10.1016/j.ymben.2021.01.007.

Library Details

Pooled Library	Name	Genes Targeted
153102	Sctrans-PP-Grp1	73
153103	Sctrans-PP-Grp2	79
153104	Sctrans-PP-Grp3	65
153105	Sctrans-PP-Grp4	84
153106	Sctrans-PP-Grp5	60
153101	Sctrans-PP	361

• Species: S. cerevisiae

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20µL
• Concentration: 25ng/µL

Resource Information

• Protocols:
  • Library NGS Protocol (29 KB)
• Depositor Data:
  • Table of gene targets, sgRNA/donor fragments, and read counts (418.1 KB)
• Terms and Licenses:
  • UBMTA

Depositor Comments

The depositing lab's raw NGS data can be downloaded from the European Nucleotide Archive (ENA): Project: PRJEB39414.