Human CRISPR Inhibition Pooled Library (Dolcetto)
(Pooled Library #1000000114, #92385, #92386)
This human CRISPR inhibition library (Dolcetto) is in backbone pXPR_050 (VP64). This Dolcetto library inhibits over 18,000 human genes and is used for genome-wide inhibition screening.
Each gene inhibited by this library is targeted by 3-6 gRNAs. These gRNAs are split among the two pools: Set A (Cat# 92385) contains gRNAs 1-3, Set B (Cat# 92386) contains gRNAs 4-6. Each pool has a unique set of gRNAs, but both pools are designed to target the same genes. The slight discrepancy in number of genes targeted between Set A and Set B is because not all genes had 6 gRNAs.
Depositor comments: Primers for the lentiGuide backbone can be used with this library in the Sequencing Protocol.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Human Dolcetto CRISPR inhibition pooled library set A was a gift from David Root and John Doench (Addgene #92385).
Human Dolcetto CRISPR inhibition pooled library set B was a gift from David Root and John Doench (Addgene #92386).
For your References section:Up, down, and out: next generation libraries for genome-wide CRISPRa, CRISPRi, and CRISPR-Cas9 knockout genetic screens. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. bioRxiv 356626. doi: 10.1101/356626. bioRxiv 356626