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Humagne Set C and Humagne Set D
(Pooled Library #172650, #172651)

  • Purpose

    The human CRISPR knockout enAsCas12a sgRNA libraries were designed for assays with limited cell numbers. Both libraries (Humagne Set C and Humagne Set D) target the same 19755 genes and each gene has one construct with 2 sgRNAs. Each library also contains 100 non-targeting pairs and 500 one-intergenic-site pairs. The total number of constructs per library is 20355. Set C contains best-ranked guides 1 and 4. Set D contains best-ranked guides 2 and 3.

  • Vector Backbone

    pRDA_052 - does not express Cas12a

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 172650 Humagne Set C 1 $500 Add to Cart
Pooled Library 172651 Humagne Set D 1 $500 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas12 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pRDA_174 (Addgene #136476) or any other plasmid(s) or cell lines expressing AsCas12a.

Library Details

  • Species
    Human
  • Genes targeted
    19,755
  • gRNAs
    40,710
  • Controls
    100 non-targeting pairs and 500 one-intergenic-site pairs in each library
  • Lentiviral Generation
    2nd

Library Shipping

These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments


Sets C and D were designed such that guides 1 and 4 were paired together (set C) and 2 and 3 (set D) rather than the sets described in the publication (1 and 2, set A) and (3 and 4, set B), which are slightly more optimal.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Knockout Humagne Set C and Humagne Set D pooled library was a gift from John Doench and David Root (Addgene #).
  • For your References section:

    Optimization of AsCas12a for combinatorial genetic screens in human cells. DeWeirdt PC, Sanson KR, Sangree AK, Hegde M, Hanna RE, Feeley MN, Griffith AL, Teng T, Borys SM, Strand C, Joung JK, Kleinstiver BP, Pan X, Huang A, Doench JG. Nature Biotech. 2020 Jul 13;39(1): 94–104. doi: 10.1038/s41587-020-0600-6. PubMed 32661438