Camargo Lab LARRY Barcode Library Version 1
(Pooled Library #140024)

• Purpose:

  The lineage and RNA recovery (LARRY) library clonally tags cells with DNA barcodes. Reading these barcodes with single cell sequencing allows for reconstruction of genome-wide transcriptional trajectories from differentiating cell populations.
  
  Each barcode is a 40-mer consisting of 28 random and 11 constant nucleotide bases with the structure:
  NNNNTGNNNNCANNNNACNNNNGANNNNGTNNNNAGNNNN

• Vector Backbone:

  pLARRY-EGFP

• Depositing Labs: Fernando Camargo
• Publication: Weinreb et al Science. 2020 Jan 23. pii: science.aaw3381. doi: 10.1126/science.aaw3381.

Library Details

• Barcode diversity: 245,979
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Barcoding Library Construction Protocol (PDF, 186 KB)
  • Library amplification, Lentivirus production, InDrop RNA barcode amplification, Genomic DNA amplification, and Library diversity testing protocols (DOC, 20 KB)
• Depositor Data:
  • Barcode diversity (XLS, 6.4 MB)
  • pLARRY-EGFP Annotated Map (GB, 15 KB)
• Terms and Licenses:
  • UBMTA

Depositor Comments

• Lineage and RNA recovery barcoding library

The depositing lab provides a script for comparing demultiplexed replicate reactions to evaluate library diversity.