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Human DECIPHER Lentiviral shRNA Libraries Mod 1 & 2
(Pooled Libraries #28285, #28286)

  • Purpose

    DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated human gene set.

    Module 2 covers disease-associated genes and drug targets that are not included in Module 1.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 28286 DECIPHER Lentiviral shRNA Library, Human Mod 2: Disease Targets 1 $450 Discontinued
Pooled Library 28285 DECIPHER Lentiviral shRNA Library, Human Mod 1: Pathway Targets Discontinued
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted

    Human Mod 1: Pathway Targets - 5043
    Human Mod 2: Disease Targets - 5412
  • shRNAs
    27,500

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼120µL
  • Concentration
    1µg/µL

Depositor Comments

DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. Human Module 2 can be used for the more comprehensive follow-up screens or simply be done in parallel. There are approximately 10,000 genes that have an Entrez/RefSeq ID and at least one interaction documented in PubMed and cover the majority of disease-associated genes and drug targets. About half of these are included in Module 1 while Module 2 covers the rest.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website for more information.

decipher-library-diagram.jpeg
How to cite this pooled library ( Back to top)

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    DECIPHER Lentiviral shRNA Library, Human Mod 2: Disease Targets was a gift from Alex Chenchik & Gus Frangou (Addgene #28286)