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Superti-Furga Lab Human SLC Activation Library
(Pooled Library #132561)

  • Purpose

    This CRISPR transcriptional activation library targets the human solute carrier family of proteins.

  • Vector Backbone

    lenti sgRNA(MS2)_zeo backbone - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 132561 gRNA pooled library 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Use in conjunction with lenti dCAS-VP64_Blast (Addgene #61425) and lenti MS2-P65-HSF1_Hygro (Addgene #61426), pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA (Addgene #113341) or any other plasmid(s) or cell lines expressing both dCas9-VP64 and MS2-p65-HSF1.

Library Details

  • Species
    Human
  • Genes targeted
    388
  • gRNAs
    3,342
  • Controls
    120 non-targeting controls
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

The library results can be analyzed with several packages, including the MAGeCK algorithms or R/DESeq.

The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human SLC Activation Library was a gift from Giulio Superti-Furga (Addgene #132561)