Skip to main content
Holiday Schedule: Addgene will be closed November 25th & 26th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about your shipment please contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Human Transcription Factor Knockout Library
(Pooled Library #162275)

  • Purpose

    This lentiviral CRISPR knockout library targets 1,639 human transcription factors for comprehensive loss-of-function screens.

  • Vector Backbone

    lentiGuide-Puro - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 162275 Lenti-human-TF-gRNA-puromycin 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Plasmid(s) such as lentiCas9-Blast (Addgene #52962) or cell lines expressing SpCas9 can be used.

Library Details

  • Species
    Human
  • Genes targeted
    1,639
  • gRNAs
    11,364
  • Controls
    100 non-targeting gRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

  • Chunliang Li lab human transcription factor CRISPR knockout library

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms and DESeq2 for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-human-TF-gRNA-puromycin library was a gift from Chunliang Li (Addgene #162275)
  • For your References section:

    Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen. Zhang H, Zhang Y, Zhou X, Wright S, Hyle J, Zhao L, An J, Zhao X, Shao Y, Xu B, Lee HM, Chen T, Zhou Y, Chen X, Lu R, Li C. Elife. 2020 Oct 1;9. pii: 57858. doi: 10.7554/eLife.57858. PubMed 33001025