Toxoplasma CRISPR Knockout Pooled Library
(Pooled Library #80636)
- PurposeToxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes.
- Vector BackbonepU6-DHFR- this backbone does not contain Cas9
- Depositing Labs
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Toxoplasma CRISPR Knockout Pooled Library was a gift from Sebastian Lourido (Addgene #80636)
For your References section:A Genome-Wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes . Sidik SM, Huet D, Ganesan SM, Huynh M, Wang T, Nasamu AS, Thiru P, Seij JPJ, Carruthers VB, Niles JC, Lourido S. Cell. 2016 doi: 10.1016/j.cell.2016.08.019. PubMed 27594426