Toronto KnockOut (TKO) CRISPR Library - Version 3
(Pooled Libraries #90294, #125517)
Human CRISPR lentiviral library that has 70,948 guides (4 guides/gene) targeting 18,053 protein-coding genes.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Toronto human knockout pooled library (TKOv3) was a gift from Jason Moffat (Addgene #)
For your References section:For #90294: Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens. Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J. G3 (Bethesda). Aug 7;7(8):2719-2727. doi: 10.1534/g3.117.041277. PubMed 28655737For #125517: Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Mair B, Tomic J, Masud SN, Tonge P, Weiss A, Usaj M, Tong AHY, Kwan JJ, Brown KR, Titus E, Atkins M, Chan KSK, Munsie L, Habsid A, Han H, Kennedy M, Cohen B, Keller G, Moffat J. Cell Rep. 2019 Apr 9;27(2):599-615.e12. doi: 10.1016/j.celrep.2019.02.041. PubMed 30970261