Morris CellTag Barcode Library
(Pooled Libraries #115643, #115644, #115645)
PurposeThese lentiviral barcoding libraries can be used to combinatorially index cells for single-cell analysis of clonal dynamics.
|Pooled Library||Minimum #
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
NOTE: For sequencing analysis, see the Morris Lab Tutorial.
Please visit https://www.biorxiv.org/content/early/2017/04/28/127860 for BioRxiv preprint.
The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CellTag Barcode library was a gift from Samantha Morris (Addgene #)
For your References section:Single-cell analysis of clonal dynamics in direct lineage reprogramming: a combinatorial indexing method for lineage tracing. Biddy BA, Kong W, Kamimoto K, Guo G, Waye SE, Sun T, Morris SA. Nature. 2018.