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Morris CellTag Barcode Library
(Pooled Libraries #115643, #115643-LVC, #115644, #115644-LVC, #115645, #115645-LVC)

  • Purpose
    These lentiviral barcoding libraries can be used to combinatorially index cells for single-cell analysis of clonal dynamics.
  • Vector Backbone
    pSMAL

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 115643 pSMAL-CellTag-V1 1 $500 Add to Cart
Lentiviral Prep 115643-LVC Virus (titer ≥ 1×108 TU/mL)
and Pooled Library DNA More Information
1 $2250 Add to Cart
Pooled Library 115644 pSMAL-CellTag-V2 1 $400 Add to Cart
Lentiviral Prep 115644-LVC Virus (titer ≥ 1×108 TU/mL)
and Pooled Library DNA More Information
1 $1700 Add to Cart
Pooled Library 115645 pSMAL-CellTag-V3 1 $400 Add to Cart
Lentiviral Prep 115645-LVC Virus (titer ≥ 1×108 TU/mL)
and Pooled Library DNA More Information
1 $1700 Add to Cart
Available to Academic and Nonprofits Only

Library Details

Pooled Library Minimum #
of Barcodes
115643 19973
115644 4934
115645 5737

  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼10µL
  • Concentration
    10ng/µL

Resource Information

Depositor Comments

NOTE: For sequencing analysis, see the Morris Lab Tutorial.

Please visit https://www.biorxiv.org/content/early/2017/04/28/127860 for BioRxiv preprint.

The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).

Information for Lentiviral Prep (Catalog # 115643-LVC) ( Back to top )

Purpose

Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.

Delivery

  • Volume 50 µl
  • Titer ≥1x108 TU/mL
  • Pricing $2000 USD for 50 µl viral preparation + $250 USD for pooled library DNA.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Pooled Library DNA (10µL at 50ng/µL) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids psPAX2 (plasmid #12260)
  • Envelope pMD2.G (plasmid #12259)
  • Buffer PBS
  • Selectable Marker EGFP

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Viral Quality Control

Pooled Library Representation:
  • To confirm library representation and distribution, we perform next-generation sequencing on the purified plasmid DNA pool that was used to generate lentivirus.

Titering Method:
  • Direct fluorescence: AAVpro 293T cells were transduced with serial dilutions of 115643-LVC. 96 hours later, GFP-positive cells were counted.

Visit our viral production page for more information.

Addgene Comments

Shipment specifications:

Pooled libraries are shipped on dry ice. Each viral service request also includes virus associated DNA, which is a sample of the purified plasmid DNA pool that was used to generate lentivirus.

How to use this virus:

Please refer to the Morris lab’s protocol for the most up to date protocol for using the Celltag libraries:

  • Brent A. Biddy, Wenjun Kong, Kenji Kamimoto, Chuner Guo, Sarah Waye, Tao Sun, Samantha Morris (2019). Single-cell mapping of lineage and identity via CellTagging. protocols.io dx.doi.org/10.17504/protocols.io.yxifxke
How to use the virus associated DNA:

Distribution of this pooled lentiviral library includes virus associated DNA, which is a sample of the purified plasmid DNA pool that was used to generate lentivirus.

  • The purified plasmid DNA pool can be amplified to make more pooled plasmid library stock DNA. Please refer to the original publication (Biddy et al., 2018), or the Morris lab’s protocol for instructions on amplifying the pooled library DNA.

Information for Lentiviral Prep (Catalog # 115644-LVC) ( Back to top )

Purpose

Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.

Delivery

  • Volume 50 µl
  • Titer ≥1x108 TU/mL
  • Pricing $1500 USD for 50 µl viral preparation + $200 USD for pooled library DNA.
  • Storage Store at -80°C. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Pooled Library DNA (10µL at 50ng/µL) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids psPAX2 (plasmid #12260)
  • Envelope pMD2.G (plasmid #12259)
  • Buffer PBS
  • Selectable Marker EGFP

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Viral Quality Control

Pooled Library Representation:
  • To confirm library representation and distribution, we perform next-generation sequencing on the purified plasmid DNA pool that was used to generate lentivirus.

Titering Method:
  • Direct fluorescence: AAVpro 293T cells were transduced with serial dilutions of 115644-LVC. 96 hours later, GFP-positive cells were counted.

Visit our viral production page for more information.

Addgene Comments

Shipment specifications:

Pooled libraries are shipped on dry ice. Each viral service request also includes virus associated DNA, which is a sample of the purified plasmid DNA pool that was used to generate lentivirus.

How to use this virus:

Please refer to the Morris lab’s protocol for the most up to date protocol for using the Celltag libraries:

  • Brent A. Biddy, Wenjun Kong, Kenji Kamimoto, Chuner Guo, Sarah Waye, Tao Sun, Samantha Morris (2019). Single-cell mapping of lineage and identity via CellTagging. protocols.io dx.doi.org/10.17504/protocols.io.yxifxke
How to use the virus associated DNA:

Distribution of this pooled lentiviral library includes virus associated DNA, which is a sample of the purified plasmid DNA pool that was used to generate lentivirus.

  • The purified plasmid DNA pool can be amplified to make more pooled plasmid library stock DNA. Please refer to the original publication (Biddy et al., 2018), or the Morris lab’s protocol for instructions on amplifying the pooled library DNA.

Information for Lentiviral Prep (Catalog # 115645-LVC) ( Back to top )

Purpose

Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.

Delivery

  • Volume 50 µl
  • Titer ≥1x108 TU/mL
  • Pricing $1500 USD for 50 µl viral preparation + $200 USD for pooled library DNA.
  • Storage Store at -80°C. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Pooled Library DNA (10µL at 50ng/µL) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids psPAX2 (plasmid #12260)
  • Envelope pMD2.G (plasmid #12259)
  • Buffer PBS
  • Selectable Marker EGFP

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Viral Quality Control

Pooled Library Representation:
  • To confirm library representation and distribution, we perform next-generation sequencing on the purified plasmid DNA pool that was used to generate lentivirus.

Titering Method:
  • Direct fluorescence: AAVpro 293T cells were transduced with serial dilutions of 115645-LVC. 96 hours later, GFP-positive cells were counted.

Visit our viral production page for more information.

Addgene Comments

Shipment specifications:

Pooled libraries are shipped on dry ice. Each viral service request also includes virus associated DNA, which is a sample of the purified plasmid DNA pool that was used to generate lentivirus.

How to use this virus:

Please refer to the Morris lab’s protocol for the most up to date protocol for using the Celltag libraries:

  • Brent A. Biddy, Wenjun Kong, Kenji Kamimoto, Chuner Guo, Sarah Waye, Tao Sun, Samantha Morris (2019). Single-cell mapping of lineage and identity via CellTagging. protocols.io dx.doi.org/10.17504/protocols.io.yxifxke
How to use the virus associated DNA:

Distribution of this pooled lentiviral library includes virus associated DNA, which is a sample of the purified plasmid DNA pool that was used to generate lentivirus.

  • The purified plasmid DNA pool can be amplified to make more pooled plasmid library stock DNA. Please refer to the original publication (Biddy et al., 2018), or the Morris lab’s protocol for instructions on amplifying the pooled library DNA.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CellTag Barcode library was a gift from Samantha Morris (Addgene #)
  • For your References section:

    Single-cell analysis of clonal dynamics in direct lineage reprogramming: a combinatorial indexing method for lineage tracing. Biddy BA, Kong W, Kamimoto K, Guo G, Waye SE, Sun T, Morris SA. Nature. 2018.