Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Parts Human Genome-Wide Reduced Double-gRNA Library
(Pooled Library #137999)

  • Purpose

    This lentiviral CRISPR knockout library enables genome-wide screens in human cell lines at reduced scale. Through optimization of guide RNA designs and delivery of two gRNAs with each construct a five-fold reduction in size is achieved without impacting performance compared with a sgRNA library with five gRNAs per target.

  • Vector Backbone

    pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 137999 Human Genome-Wide Reduced Double-gRNA library 1 $500 Pending
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or other plasmids or cell lines expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    19,657
  • gRNAs
    59,576
  • Controls
    398 non-targeting controls
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

bioRxiv pre-print: https://www.biorxiv.org/content/10.1101/859652v1
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Reduced Double-gRNA Library was a gift from Leopold Parts (Addgene #137999)
  • For your References section:

    Minimized double guide RNA libraries enable scale-limited CRISPR/Cas9 screens. Peets EM, Crepaldi L, Zhou Y, Allen F, Elmentaite R, Noell G, Turner G, Iyer V, Parts L. bioRxiv https://www.biorxiv.org/content/10.1101/859652v1. BioRxiv 859652v1