Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Pu Lab - Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
(Pooled Library #138015)

  • Purpose

    This AAV CRISPR knockout library is designed to investigate cardiac gene regulation.

    Guide RNAs target transcriptional regulators expressed in mouse cardiomyocytes and a subset of GATA4 regulated genes.

  • Vector Backbone

    AAV-U6-gRNA-TnT-Cre - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 138015 gRNA pooled library 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item. The AAV backbone expresses a Cre-recombinase and can be used with Cre-activatable Cas9.

Library Details

  • Species
    Mouse
  • Genes targeted
    2,444
  • gRNAs
    14,671
  • Controls
    7 gRNAs target human genome

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    25ng/µL

Depositor Comments

Mouse AAV CRISPR Knockout Library targeting cardiac transcriptional regulators
  • Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library was a gift from William Pu (Addgene #138015)
  • For your References section:

    In vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation. VanDusen N.J., Lee J.Y., Gu W., Sethi I., Zheng Y., King J.S., Zhou P., Suo S., Guo Y., Ma Q., Yuan G., Pu W.T. bioRxiv 808402v1 https://www.biorxiv.org/content/10.1101/808402v1.full.