Human Activity-Optimized CRISPR Knockout Library (3 sub-libraries in lentiCRISPRv1)
(Pooled Library #1000000100)
The Sabatini/Lander CRISPR pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.
The library is split into three sub-libraries. Two tubes each contain 90,000 gRNAs each or 5 sgRNAs per gene. The third tube contains 5,000 gRNAs.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Activity-Optimized Human CRISPR Pooled Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000100)
For your References section:Identification and characterization of essential genes in the human genome. Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM. Science. 2015 Oct 15. pii: aac7041. PubMed 26472758