Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools)
(Pooled Libraries #51043, #51044, #51045, #51046, #51047, #51048)
Human CRISPR knockout pooled libraries that have been separated into 6 distinct, enriched sub-pools.
Note that while each sub-pool is enriched for their respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
- Vector BackbonepLX-sgRNA- this backbone does not contain Cas9
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Human CRISPR enriched pooled library was a gift from David Sabatini & Eric Lander (Addgene # )
For your References section:Genetic screens in human cells using the CRISPR-Cas9 system . Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. 10.1126/science.1246981PubMed 24336569