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Wei Human lncRNA Splicing-targeting CRISPR Library
(Pooled Library #119977)

  • Purpose

    This CRISPR library is designed to target the 5' or 3' splice sites of lncRNAs in genome-scale to knockout the targeted lncRNAs.

  • Vector Backbone

    pLenti-sgRNA(lib)-puro - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 119977 gRNA pooled library 1 $400 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with cell lines expressing Cas9.

Library Details

  • Species
    Human
  • lncRNAs targeted
    10,996
  • gRNAs
    126,773
  • Controls
    500 non-targeting, 350 ribosomal gene-targeting
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

The read distribution of the two biologically independent samples of the control library in K562 cells showed a high level of correlation (Spearman Corr. = 0.94). Sequencing data from the CRISPR screen can be accessed in NCBI Short Read Archive (SRA) with the accession number SRP157958 under BioProject ID PRJNA486076.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human lncRNA Splicing-targeting CRISPR library was a gift from Wensheng Wei (Addgene #119977)
  • For your References section:

    Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W. Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283. PubMed 30395134