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Human paired-guide RNA (pgRNA) Library for long non-coding RNAs (lncRNAs)
(Pooled Library #89640)

  • Purpose

    This library is designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).

  • Vector Backbone

    Modified scaffold-linker-U6 - this backbone does not contain Cas9

    Backbone modification includes second U6 insertion. View sequencing result here.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 89640 Human pgRNA library lncRNAs (long non-coding RNAs) 1 $400 Add to Cart
This material is available to academics and nonprofits only.
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately.

Library Details

  • Species
    Human
  • Genes targeted
    671 long non-coding RNAs (lncRNAs)
  • gRNAs
    12,472 pairs
  • Controls
    2000 positive; 500 negative
  • Lentiviral Generation
    3rd

Library Shipping

This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

You will receive:

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    pgRNA CRISPR-Cas9 Library for human non-coding RNAs (lncRNAs) was a gift from Wensheng Wei (Addgene # 89640).
  • For your References section:

    Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W. Nature. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. PubMed 27798563