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Human Subpooled CRISPRi-v2 Libraries
(Pooled Libraries #83971, #83972, #83973, #83974, #83975, #83976, #83977)

  • Purpose

    Version 2 - Human subpooled CRISPRi libraries for inhibition of gene transcription. Available as 5 gRNAs per gene.

  • Vector Backbone

    pCRISPRia-v2 - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 83971
  • Kinases, Phosphatases, and Drug Targets - gRNA pooled library

1 $400 Add to Cart
Pooled Library 83972
  • Cancer and Apoptosis - gRNA pooled library

1 $400 Add to Cart
Pooled Library 83973
  • Stress and Proteostasis - gRNA pooled library

1 $400 Add to Cart
Pooled Library 83974
  • Mitochondria, Trafficking, and Motility - gRNA pooled library

1 $400 Add to Cart
Pooled Library 83975
  • Gene expression - gRNA pooled library

1 $400 Add to Cart
Pooled Library 83976
  • Membrane Proteins - gRNA pooled library

1 $400 Add to Cart
Pooled Library 83977
  • Unassigned - gRNA pooled library

1 $400 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB (#46911) or otherwise used with cell lines already expressing inhibitory dCas9.

Library Details

Pooled Library Genes Targeted Targeting gRNAs Control gRNAs
83971 2318 12,775 250
83972 2916 16,045 280
83973 3093 16,615 290
83974 2219 12,050 250
83975 2292 12,465 250
83976 2418 12,955 250
83977 3649 19,735 325
  • Species
    Human
  • Lentiviral Generation
    3rd

Library Shipping

These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

  • Volume
    20 µL
  • Concentration
    12.5 ng/µL

Depositor Comments

In addition to dCas9-KRAB (#46911), another dCas9-containing construct that can be used with these libraries is pHR-SFFV-KRAB-dCas9-P2A-mCherry (#60954).

The data analysis pipeline for analyzing sequencing data is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #)
  • For your References section:

    Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255