Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Wright Human Membrane Protein Activation Library
(Pooled Library #113345)

  • Purpose

    This lentiviral CRISPR activation library targets the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform. It can be used for large-scale screening for receptor identification, antibody cross-reactivity assessments, or characterization of extracellular interactions.

  • Vector Backbone

    pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 113345 gRNA pooled library 1 $500 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA (Addgene #113341) or any other plasmid(s) or cell lines expressing both dCas9-VP64 and MS2-p65-HSF1.

Library Details

  • Species
    Human
  • Genes targeted
    6,213
  • gRNAs
    58,570
  • Controls
    500 non-targeting
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Membrane Protein Activation library was a gift from Gavin Wright (Addgene #113345)
  • For your References section:

    Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Chong ZS, Ohnishi S, Yusa K, Wright GJ. Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. PubMed 30477585