Human CRISPR Activation Library (SAM - 2 plasmid system)
(Pooled Library #1000000078)
Version 2 of the human CRISPR/Cas9 SAM (Synergistic Activation Mediator) pooled library. This library uses an engineered protein complex for the transcriptional activation of endogenous genes.
For SAM gain-of-function screening, this gRNA library has to be combined with 1 additional SAM construct, MS2-P65-HSF1 (Addgene plasmid #89308), which is provided with the library.
- Vector BackbonelentiSAMv2- this backbone contains dCas9-VP64-Blast and sgRNA(MS2)
Note: Libraries ordered prior to 4/3/2017 were shipped with Addgene plasmid #61426 rather than #89308
- Depositing Labs
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Human CRISPR 2-plasmid activation pooled library (SAM) was a gift from Feng Zhang (Addgene # 1000000078)
For your References section:Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, & Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed 28333914