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7291 Results

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Plasmids, Strains, and Pooled Libraries 1–20 of 7291

  1. Plasmid
    #117686
    Depositor
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Insert
    eSpCas9(1.1) Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
  2. Plasmid
    #104873
    Depositor
    Purpose
    HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts
    Insert
    T2A-QF2 Budding Yeast, Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Insect
    Mutation
    None
  3. Plasmid
    #126882
    Depositor
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Insert
    Wheat_live_Cas9 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  4. Plasmid
    #113656
    Depositor
    Purpose
    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.
    Insert
    Native promoter and dCas9
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  5. Plasmid
    #113654
    Depositor
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Insert
    promoter J23119 and sgRNA
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  6. Plasmid
    #104874
    Depositor
    Purpose
    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.
    Insert
    T2A-GAL80 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Insect
    Mutation
    None
  7. Plasmid
    #126881
    Depositor
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Insert
    Wheat_live_Cas9 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  8. Plasmid
    #113655
    Depositor
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Insert
    promoter T5 and sgRNA
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  9. pSL2680 Empty backbone
    Plasmid
    #85581
    Depositor
    Purpose
    Replicating base vector for constructing cpf1/CRISPR editing plasmids
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
  10. Plasmid
    #113657
    Depositor
    Purpose
    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.
    Insert
    TDK gene promoter and dCas9
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  11. Plasmid
    #47319
    Depositor
    Purpose
    Cas9m4 Activator
    Insert
    Cas9m4-VP64
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  12. Plasmid
    #46760
    Depositor
    Insert
    egfp target Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  13. Plasmid
    #49771
    Depositor
    Purpose
    Level 1 hCas9 module
    Insert
    35Sp::hCas9 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  14. Plasmid
    #47314
    Depositor
    Purpose
    Cas9 D10A+H840A
    Insert
    Cas9m2
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  15. Plasmid
    #121194
    Depositor
    Purpose
    C-terminal tagging
    Insert
    mAID-mCherry-BSD Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  16. Plasmid
    #115630
    Depositor
    Purpose
    expression esgRNA in wheat protoplasts
    Insert
    TaU6p-esgRNA
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  17. Plasmid
    #104171
    Depositor
    Purpose
    Cas9-WT mammalian expression
    Insert
    Cas9-WT
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    WT
  18. Plasmid
    #53373
    Depositor
    Purpose
    wildtype Cas9 expression plasmid
    Insert
    Cas9
    Use
    CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  19. Plasmid
    #101153
    Depositor
    Purpose
    gRNA with 14 MCP binding site
    Insert
    MUC4 Human
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mutation
    None
  20. Plasmid
    #91032
    Depositor
    Purpose
    Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP
    Insert
    TaCas9 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
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