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8922 Results

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Plasmids, Strains, and Pooled Libraries 1–20 of 8922

  1. Plasmid
    #12259
    Depositor
    Purpose
    VSV-G envelope expressing plasmid
    Insert
    VSV G
    Use
    Lentiviral ; Envelope
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  2. psPAX2 Empty backbone
    Plasmid
    #12260
    Depositor
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  3. Plasmid
    #52961
    Depositor
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Insert
    Cas9 Synthetic
    Insert
    Puromycin resistance
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  4. Plasmid
    #60903
    Depositor
    Purpose
    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation
    Insert
    Cas9 dead
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  5. Plasmid
    #52963
    Depositor
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Insert
    S. pyogenes sgRNA cassette Synthetic
    Insert
    Puromycin resistance
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  6. Plasmid
    #44385
    Depositor
    Insert
    mitoGFP Human
    Use
    Lentiviral
    Tags
    Cox8 targeting sequence, GFP
    Expression
    Mammalian
    Mutation
    None
  7. pLV-mCherry Empty backbone
    Plasmid
    #36084
    Depositor
    Purpose
    3rd generation lentiviral plasmid expressing mCherry
    Insert
    mCherry Synthetic
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  8. Plasmid
    #52962
    Depositor
    Viral service
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Insert
    Cas9 Synthetic
    Insert
    Blasticidin resistance
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  9. Plasmid
    #109049
    Depositor
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting
    Insert
    CasRx Human; Ruminococcus flavefaciens XPD3002
    Use
    Lentiviral, CRISPR
    Tags
    NLS, HA, 2A-eGFP
    Expression
    Mammalian
    Mutation
    None
  10. Plasmid
    #99154
    Depositor
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Insert
    Cas9 Synthetic
    Insert
    mCherry Discoma sp.
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  11. Plasmid
    #71237
    Depositor
    Purpose
    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector
    Insert
    humanized dCas9-KRAB T2A GFP S. Pyogenes
    Insert
    sgRNA
    Use
    Lentiviral, CRISPR
    Tags
    Flag
    Expression
    Mutation
    D10A and H840A
  12. Plasmid
    #12251
    Depositor
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  13. Plasmid
    #10879
    Depositor
    Insert
    non-hairpin 18bp
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  14. Plasmid
    #80921
    Depositor
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Use
    Lentiviral ; Doxycycline indu...
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  15. pLV-EF1a-IRES-Blast Empty backbone
    Plasmid
    #85133
    Depositor
    Purpose
    lentiviral backbone
    Use
    Lentiviral
    Tags
    None
    Expression
    Mutation
    None
  16. Plasmid
    #46911
    Depositor
    Purpose
    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
    Insert
    dCas9-BFP-KRAB fusion Human
    Use
    Lentiviral, CRISPR
    Tags
    KRAB domain, BFP, HA, 2xNLS
    Expression
    Mammalian
    Mutation
    None
  17. Plasmid
    #71236
    Depositor
    Purpose
    Express sgRNA and dCas9-KRAB from lentiviral vector
    Insert
    humanized dCas9-KRAB T2A Puro S. Pyogenes
    Insert
    sgRNA
    Use
    Lentiviral, CRISPR
    Tags
    Flag
    Expression
    Mutation
    D10A and H840A
  18. Tet-pLKO-puro Empty backbone
    Plasmid
    #21915
    Depositor
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Use
    Lentiviral
    Tags
    None
    Expression
    Mutation
    None
  19. Plasmid
    #84832
    Depositor
    Purpose
    CRISPRi/a V2 library parental plasmid
    Insert
    sgRNA
    Insert
    puro-T2A-BFP
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  20. pLKO.3G Empty backbone
    Plasmid
    #14748
    Depositor
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
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