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8788 Results

Your Selections:

Plasmids, Strains, and Pooled Libraries 1–20 of 8788

  1. Plasmid
    #52961
    Depositor
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Insert
    Cas9 Synthetic
    Insert
    Puromycin resistance
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  2. Plasmid
    #12259
    Depositor
    Purpose
    VSV-G envelope expressing plasmid
    Insert
    VSV G
    Use
    Lentiviral ; Envelope
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  3. psPAX2 Empty backbone
    Plasmid
    #12260
    Depositor
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  4. Plasmid
    #1864
    Depositor
    Purpose
    3rd gen lentiviral negative control vector containing scrambled shRNA
    Insert
    scramble
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  5. pLKO.1 puro Empty backbone
    Plasmid
    #8453
    Depositor
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  6. pLJM1-EGFP Empty backbone
    Plasmid
    #19319
    Depositor
    Purpose
    3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin
    Insert
    none
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
  7. Plasmid
    #87360
    Depositor
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Insert
    Cas9-2A-eGFP
    Use
    Lentiviral, CRISPR ; Doxycycl...
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    None
  8. pLKO.1 hygro Empty backbone
    Plasmid
    #24150
    Depositor
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  9. pLX304 Empty backbone
    Plasmid
    #25890
    Depositor
    Purpose
    3rd generation lentiviral vector
    Use
    Lentiviral ; Gateway Destinat...
    Tags
    V5
    Expression
    Mammalian
    Mutation
    None
  10. Plasmid
    #12253
    Depositor
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Insert
    Rev ( rev )
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  11. Plasmid
    #12251
    Depositor
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  12. Plasmid
    #99373
    Depositor
    Purpose
    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)
    Insert
    (Sp)dCas9-VPR-P2A-Puro Human, Mouse, Synthetic; ; S. pyogenes
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  13. pLKO.1-blast Empty backbone
    Plasmid
    #26655
    Depositor
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  14. Plasmid
    #52963
    Depositor
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Insert
    S. pyogenes sgRNA cassette Synthetic
    Insert
    Puromycin resistance
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  15. Plasmid
    #109049
    Depositor
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting
    Insert
    CasRx Human; Ruminococcus flavefaciens XPD3002
    Use
    Lentiviral, CRISPR
    Tags
    2A-eGFP, HA, NLS
    Expression
    Mammalian
    Mutation
    None
  16. Plasmid
    #10878
    Depositor
    Purpose
    3rd generation transfer plasmid
    Insert
    stuffer
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  17. Plasmid
    #86849
    Depositor
    Purpose
    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection
    Insert
    mKO2-hCDT1(30-120) Synthetic
    Insert
    mAG-hGEM(1-110) Synthetic
    Insert
    pac (puromycin N-acetyl-transferase) S. alboniger
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  18. Plasmid
    #109050
    Depositor
    Purpose
    Catalytically inactive dCasRx with 2A-EGFP
    Insert
    dCasRx Human; Ruminococcus flavefaciens XPD3002
    Use
    Lentiviral, CRISPR
    Tags
    2A-eGFP, HA, NLS
    Expression
    Mammalian
    Mutation
    R239A/H244A/R858A/H863A
  19. Plasmid
    #26429
    Depositor
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  20. Plasmid
    #85140
    Depositor
    Purpose
    Lentiviral expression of hTERT, used to create immortalized cell lines
    Insert
    hTERT ( TERT Human )
    Use
    Lentiviral
    Tags
    None
    Expression
    Mutation
    None
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