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8619 Results

Your Selections:

Plasmids, Strains, and Pooled Libraries 1–20 of 8619

  1. Plasmid
    #52963
    Depositor
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Insert
    S. pyogenes sgRNA cassette Synthetic
    Insert
    Puromycin resistance
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  2. psPAX2 Empty backbone
    Plasmid
    #12260
    Depositor
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  3. Plasmid
    #12259
    Depositor
    Purpose
    VSV-G envelope expressing plasmid
    Insert
    VSV G
    Use
    Lentiviral ; Envelope
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  4. Plasmid
    #52961
    Depositor
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Insert
    Cas9 Synthetic
    Insert
    Puromycin resistance
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  5. Plasmid
    #1864
    Depositor
    Purpose
    3rd gen lentiviral negative control vector containing scrambled shRNA
    Insert
    scramble
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  6. Plasmid
    #12251
    Depositor
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  7. Plasmid
    #109049
    Depositor
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting
    Insert
    CasRx Human; Ruminococcus flavefaciens XPD3002
    Use
    Lentiviral, CRISPR
    Tags
    NLS, HA, 2A-eGFP
    Expression
    Mammalian
    Mutation
    None
  8. Plasmid
    #52962
    Depositor
    Viral service
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Insert
    Cas9 Synthetic
    Insert
    Blasticidin resistance
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  9. Plasmid
    #73310
    Depositor
    Purpose
    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter
    Insert
    Cas9 Synthetic
    Use
    Lentiviral, CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    None
  10. Plasmid
    #22945
    Depositor
    Purpose
    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag
    Insert
    tumor protein 53 ( TP53 Human )
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    None
  11. pLKO.1 puro Empty backbone
    Plasmid
    #8453
    Depositor
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Use
    Lentiviral, RNAi
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  12. Plasmid
    #66803
    Depositor
    Purpose
    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector
    Insert
    IDH1 ( IDH1 Human )
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R132H
  13. Plasmid
    #17452
    Depositor
    Purpose
    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro
    Use
    Lentiviral ; Destination vector
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  14. pLenti-puro Empty backbone
    Plasmid
    #39481
    Depositor
    Purpose
    Tetracycline inducible 3rd generation lentiviral vector
    Use
    Lentiviral
    Tags
    V5 and 6XHis
    Expression
    Mammalian
    Mutation
    None
  15. Plasmid
    #61425
    Depositor
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast Synthetic; S. pyogenes
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
  16. Plasmid
    #12253
    Depositor
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Insert
    Rev ( rev )
    Use
    Lentiviral ; Packaging
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  17. Plasmid
    #50661
    Depositor
    Purpose
    Doxycycline-inducible lentiviral expression of SpCas9
    Insert
    humanized S. pyogenes Cas9
    Use
    Lentiviral, CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    None
  18. pLV-EF1a-IRES-Puro Empty backbone
    Plasmid
    #85132
    Depositor
    Purpose
    lentiviral backbone
    Use
    Lentiviral
    Tags
    None
    Expression
    Mutation
    None
  19. Plasmid
    #17446
    Depositor
    Purpose
    3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro
    Insert
    GFP
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  20. FU-tetO-Gateway Empty backbone
    Plasmid
    #43914
    Depositor
    Use
    Lentiviral ; Gateway Destinat...
    Tags
    V5, 6xHis
    Expression
    Mammalian
    Mutation
    None
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