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Showing: 21 - 40 of 108 results
  1. FREQ-Seq Kit

    Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  2. Hamdoun lab FP fusion plasmids

    Kit
    #1000000033
    Purpose
    Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  3. Musunuru-Cowan TALEN Kit

    Kit
    #1000000034
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  4. Endy/Shearwin pOSIP plasmid set

    Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  5. GreenGate

    Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  6. BIOFAB

    Kit
    #1000000037
    Purpose
    Sets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interest
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  7. Ekker Lab TALEN kit

    Kit
    #1000000038
    Purpose
    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  8. FX cloning Kit

    Kit
    #1000000039
    Purpose
    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  9. Platinum Gate TALEN Kit

    Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  10. MoClo Tool Kit

    Kit
    #1000000044
    Purpose
    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators
    Availability
    Academic Institutions and Nonprofits only
  11. MultiSite Gateway plasmid toolKit

    Kit
    #1000000050
    Purpose
    Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  12. PUF Assembly Kit

    Kit
    #1000000051
    Purpose
    Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  13. Golden GATEway Cloning Kit

    Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  14. Multiplex CRISPR/Cas9 Assembly System Kit

    Kit
    #1000000055
    Purpose
    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  15. pCri System

    Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Availability
    Academic Institutions and Nonprofits only
  16. CIDAR MoClo Parts Kit

    Kit
    #1000000059
    Purpose
    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  17. Multiple Lentiviral Expression (MuLE) system

    Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  18. MoClo Yeast Toolkit

    Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  19. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Kit
    #1000000062
    Purpose
    Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  20. FusX TALEN assembly system

    Kit
    #1000000063
    Purpose
    Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics
    Depositor
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 108 results