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Addgene
Showing: 61 - 80 of 6905 results

  5. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Article
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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  6. pBS7

    Plasmid
    #83774
    Purpose
    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Venus
    Use
    Tags
    Venus
    Expression
    Yeast
    Mutation
    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83774-plasmid-map-sequence-id-159984

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  7. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

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  8. pDH6

    Plasmid
    #83777
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y mutations added.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83777-plasmid-map-sequence-id-160009

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  9. pDH3

    Plasmid
    #83789
    Purpose
    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    CFP
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    F64L, Y66W, N146I, M153T, V163A, N164H mutations …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83789-plasmid-map-sequence-id-160016

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Showing: 61 - 80 of 6905 results