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Addgene
Showing: 61 - 80 of 67999 results

  1. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid
    #105810
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105810-plasmid-map-sequence-id-204854

    Enlarge

  2. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid
    #105811
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105811-plasmid-map-sequence-id-204856

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  3. pKK-RNAi-nucEGFPmiR-TEV-mCherry

    Plasmid
    #105812
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105812-plasmid-map-sequence-id-203423

    Enlarge

  4. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid
    #105813
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105813-plasmid-map-sequence-id-204858

    Enlarge

  5. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

    Plasmid
    #105814
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105814-plasmid-map-sequence-id-203426

    Enlarge

  10. SP65U1-135a-2

    Plasmid
    #105820
    Purpose
    plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid
    Depositor
    Cecilia Mannironi
    Insert
    MIRNA135A2 (Mir135a-2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1
    Availability
    Academic Institutions and Nonprofits only
    105820-plasmid-map-sequence-id-202522

    Enlarge

  11. SP65U1-301a

    Plasmid
    #105821
    Purpose
    plasmid for the overexpression of miR-301a under the U1 expression cassette inserted in the pSP65 plasmid
    Depositor
    Cecilia Mannironi
    Insert
    MIRNA301 (Mir301 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1
    Availability
    Academic Institutions and Nonprofits only
    105821-plasmid-map-sequence-id-202521

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  17. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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Showing: 61 - 80 of 67999 results