Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Showing: 1 - 20 of 17433 results
  1. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Use
    Tags
    IRES, TEV, and GST
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    tac and IRES
    Availability
    Academic Institutions and Nonprofits only
  2. psbA2-PHLS (b)

    Plasmid
    #52307
    Purpose
    Replaces the psbA2 gene in Synechocystis with the PHLS gene
    Depositor
    Inserts
    beta-phellandrene synthase
    Chloramphenicol resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    psbA2
    Availability
    Academic Institutions and Nonprofits only
  3. psbA2(noATbox)-PHLS (c)

    Plasmid
    #52308
    Purpose
    Replaces the psbA2 gene in Synechocystis with the PHLS gene
    Depositor
    Inserts
    beta-phellandrene synthase
    Chloramphenicol resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    CAAATACA box in the psbA2 promoter to ggcgcgcc
    Promoter
    psbA2
    Availability
    Academic Institutions and Nonprofits only
  4. psbA2-Ptrc-PHLS (d)

    Plasmid
    #52309
    Purpose
    Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene
    Depositor
    Inserts
    beta-phellandrene synthase
    Chloramphenicol resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptrc
    Availability
    Academic Institutions and Nonprofits only
  5. cpc-PHLS (g)

    Plasmid
    #52311
    Purpose
    Replaces the cpc operon in Synechocystis with the PHLS gene
    Depositor
    Inserts
    beta-phellandrene synthase
    Chloramphenicol resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synechocystis endogenous cpc-operon promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, MBP, TEV, and His
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  7. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Insert
    monomeric streptavidin 2
    Use
    Tags
    TEV site, Thioredoxin, S tag, FLAG, and His
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  8. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    Agrobacterium
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pFGL815N

    Plasmid
    #52323
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    Agrobacterium
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 17433 results