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6,024 results

Showing: 41 - 60 of 6024 results

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pYEXC3H

Plasmid

#49026

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

Enlarge
pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-12Pk-kanMX6

Plasmid

#49176

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

12Pk

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-12Pk-hphMX6

Plasmid

#49177

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

12Pk

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-12Pk-LEU2MX6

Plasmid

#49179

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

12Pk

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-12Pk-ura4MX6

Plasmid

#49180

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

12Pk

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-G9-5FLAG-kanMX6

Plasmid

#49181

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

G9-5FLAG

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-G11-5FLAG-kanMX6

Plasmid

#49182

Purpose

genomic G11-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

G11-5FLAG

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-mRFP-ura4MX6

Plasmid

#49183

Purpose

genomic mRFP tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

mRFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-ura4MX6

Plasmid

#49184

Purpose

gene deletion in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-G9-5FLAG-hphMX6

Plasmid

#49185

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

G9-5FLAG

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only
GFP-ATG8(414)/GFP-AUT7(414)

Plasmid

#49424

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

Depositor

Daniel Klionsky

Article

Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

ATG8

Availability

Academic Institutions and Nonprofits only
GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

ATG8

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH62-EBD

Plasmid

#49455

Purpose

yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase

Depositor

Marc Gartenberg

Article

Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

Insert

Cre-EBD fusion

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Gal1 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-recR

Plasmid

#49457

Purpose

single copy recombinase yeast expression plasmid

Depositor

Marc Gartenberg

Article

Ansari et al Methods. 1999 Feb;17(2):104-11.

Insert

recR

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC6-TEFkFLP(LYS2)

Plasmid

#49458

Purpose

overespression of Flp step-arrest mutant in yeast, LYS2 selection

Depositor

Marc Gartenberg

Insert

Flp(H305L) (flp Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIS-GALkFLP(Trp1)

Plasmid

#49459

Purpose

overespression of Flp step-arrest mutant in yeast, TRP1 selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overespression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Availability

Academic Institutions and Nonprofits only

Enlarge
GBK-Rab1AQ70LdeltaCC

Plasmid

#49555

Purpose

expresses GAL4BD-Rab1AQ70Ldelta CC in yeast

Depositor

Marci Scidmore

Article

Cortes et al Infect Immun. 2007 Dec;75(12):5586-96. E

Insert

Rab1AQ70L (RAB1A Human)

Use

Tags

Gal4 Binding Domain and Myc

Expression

Yeast

Mutation

Glutamine 70 to Leucine, deleted amino acids Cyst…

Promoter

ADHI

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 41 - 60 of 6024 results

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