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Showing: 1 - 20 of 7916 results

  2. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    52628-plasmid-map-sequence-id-217983

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  3. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    Nuclear localization signal and 3X FLAG
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    52694-plasmid-map-sequence-id-82051

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  6. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and Ef1-a
    52963-plasmid-map-sequence-id-219854

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Showing: 1 - 20 of 7916 results