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Showing: 1 - 20 of 9582 results

  2. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    52628-plasmid-map-sequence-id-217983

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  3. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    Nuclear localization signal and 3X FLAG
    Expression
    Mutation
    Promoter
    Toxoplasma U6 and TgTUB1
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  4. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    Cas9
    Puromycin resistance
    Use
    Lentiviral and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  5. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    Lentiviral and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

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  6. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and Ef1-a
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  7. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    NLS and Fok1 nuclease (homodimeric wildtype)
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

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  16. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Availability
    Academic Institutions and Nonprofits only
    49013-plasmid-map-sequence-id-231494

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Showing: 1 - 20 of 9582 results