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Showing: 1 - 15 of 15 results
  1. pcDNA3-CKAR2

    Plasmid
    #123348
    Purpose
    Improved FRET-based biosensor for monitoring Protein Kinase C activity.
    Depositor
    Insert
    CKAR2
    Use
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid
    #123343
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Insert
    Venus-cpVenus-FLARE-CKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    PKCsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-mCherry-PKCsub-RLuc8N-NES

    Plasmid
    #138213
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Insert
    mCherry-PKCsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid
    #138299
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Insert
    mCherry-PKCsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 15 of 15 results