Addgene

We narrowed to 20 results for: Jin Zhang ekar

Showing: 1 - 20 of 20 results

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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cytoEKAR4

Plasmid

#174438

Purpose

Cytosol-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

cytoEKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Lyn-FLINC-EKAR1

Plasmid

#87707

Purpose

Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Lyn-FLINC-EKAR1

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Lyn-FLINC-EKAR1(TA)

Plasmid

#87708

Purpose

Negative-control mutant of Lyn-FLINC-EKAR1.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Lyn-FLINC-EKAR1(TA)

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-EKAR4

Plasmid

#205513

Purpose

Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-EKAR4

Use

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Use

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-RAB-EKARev

Plasmid

#118477

Purpose

ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-RAB-EKARev

Use

Tags

N-terminal targeting sequence from Lyn-kinase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

Plasmid

#123339

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al Elife. 2018 Jul 3;7. pii: 35458

Insert

Venus-cpVenus-FLARE-EKAR-EV

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

Plasmid

#123340

Purpose

Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

Depositor

Jin Zhang

Article

Ross et al Elife. 2018 Jul 3;7. pii: 35458

Insert

Venus-cpVenus-FLARE-EKAR-EV(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

Plasmid

#123341

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al Elife. 2018 Jul 3;7. pii: 35458

Insert

mCherry-mCherry-FLARE-EKAR-EV

Use

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

Plasmid

#123342

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al Elife. 2018 Jul 3;7. pii: 35458

Insert

Cerulean3-Cerulean3-FLARE-EKAR-EV

Use

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsubDD-Venus-NES

Plasmid

#84630

Purpose

Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsubDD-Venus-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsub(TA)DD-Venus-NES

Plasmid

#84631

Purpose

Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsub(TA)DD-Venus-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Mutated target Thr residue to Ala.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge