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Let7-IRE-TREAT

Plasmid

#196925

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE-let7-SunTag-TREAT

Plasmid

#196926

Purpose

SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE-let7R-SunTag-TREAT

Plasmid

#196927

Purpose

SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Let7-SunTag

Plasmid

#196928

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Let7R-SunTag

Plasmid

#196929

Purpose

Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
miR21-IRE-TREAT

Plasmid

#196930

Purpose

mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
miR21mutant-IRE-TREAT

Plasmid

#196931

Purpose

Mutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE-miR21-SunTag-TREAT

Plasmid

#196932

Purpose

SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE-miR21mutant-SunTag-TREAT

Plasmid

#196933

Purpose

SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Let7R-IRE-TREAT

Plasmid

#196934

Purpose

Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
RIP7-RLuc-YFP-pBS

Plasmid

#72482

Purpose

Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

Depositor

Vincent Poitout

Article

Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

Inserts

rat ins2 gene promoter (Ins2 Rat)
luciferase
eYFP

Use

Luciferase

Tags

eYFP and renilla luciferase

Expression

Mammalian

Mutation

eYFP varient

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-6-RLuc-9

Plasmid

#197739

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Renilla luciferase (tomato optimized)

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pscAAV-CAG-RLuc

Plasmid

#83280

Purpose

recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

Insert

Renilla Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH727-CEN-RLuc/staCFLuc

Plasmid

#38211

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH743-CEN-RLuc/slowstaCFLuc

Plasmid

#38223

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192387

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP744_L2pV1_OR_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192388

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP745_L2pV1_OR_B3RT_FRT_Rluc_1IB_lacZ

Plasmid

#192389

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP746_L2pV1_OR_B3RT_FRT_Rluc_2IFB_lacZ

Plasmid

#192390

Purpose

To act as a 2-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge